Sustained delivery of transforming growth factor beta three enhances tendon-to-bone healing in a rat model

摘要: Despite advances in surgical technique, rotator cuff repairs are plagued by a high rate of failure. This failure is part due to poor tendon-to-bone healing; rather than regeneration fibrocartilaginous attachment, the repair filled with disorganized fibrovascular (scar) tissue. Transforming growth factor beta 3 (TGF-β3) has been implicated fetal development and scarless healing and, thus, exogenous addition TGF-β3 may enhance healing. We hypothesized that: could be released controlled manner using heparin/fibrin-based delivery system (HBDS); at insertion would lead improvements biomechanical properties compared untreated controls. After demonstrating that release kinetics HBDS vitro, matrices were incorporated repaired supraspinatus insertions rats. Animals sacrificed 14-56 days. Repaired assessed histology (for inflammation, vascularity, cell proliferation) biomechanics structural mechanical properties). treatment vivo accelerated process, increases cellularity, proliferation early timepoints. Moreover, sustained led significant 28 days material 56 concluded delivered enhanced rat model.