Cutting Edge: Functional Role for Proline-Rich Tyrosine Kinase 2 in NK Cell-Mediated Natural Cytotoxicity

摘要: Protein tyrosine kinase activation is one of the first biochemical events in signaling pathway leading to NK cell cytolytic machinery. Here we investigated whether proline-rich 2 (Pyk2), nonreceptor protein belonging focal adhesion family, could play a role cell-mediated cytotoxicity. Our results demonstrate that binding cells sensitive target or ligation beta2 integrins rapid induction Pyk2 phosphorylation and activation. By contrast, no detectable found upon CD16 stimulation mediated by either mAb interaction with Ab-coated P815 cells. A functional for natural but not Ab-mediated cytotoxicity was demonstrated use recombinant vaccinia viruses encoding dead mutant Pyk2. Finally, provide evidence involved integrin-triggered extracellular signal-regulated activation, supporting hypothesis plays controlling