Specific Th1 cytokine down-regulation associated with primary clinically derived human immunodeficiency virus type 1 Nef gene-induced expression.

摘要: HIV-1 infection is associated with a progressive and functional decline in the CD4+ lymphoid Th1 subset. Here, we propose that HIV nef gene product may function as specific regulator of cytokine production. By use T cell-specific inducible expression system, show upon cell activation, induced down-regulated both IL-2 IFN-gamma production dose-dependent manner, whereas IL-4, IL-9, IL-13, IL-8, TNF-alpha remained unaffected. In addition to this, independent transfected clones expressing various genes, including sequences amplified directly from an primary clinical isolate, displayed similar pattern expression. The impairment by nef, therefore, seems be important conserved feature represent significant this viral AIDS pathogenesis.