Effects of Egr1 on pancreatic acinar intracellular trypsinogen activation and the associated ceRNA network.

摘要: Acute pancreatitis (AP) is a common digestive disorder with high morbidity and mortality. The present study aimed to investigate the expression of early growth response protein 1 (Egr1), effect competing endogenous (ce)RNA network on trypsinogen activation. Pancreatic acinar intracellular activation (PAITA) an important event in stage AP; however, underlying mechanisms remain unclear. used taurolithocholic acid 3‑sulfate (TLC‑S)‑treated AR42J cells (pancreatic cell line) establish PAITA model. A gene microarray bioinformatics analysis was performed identify potential key targets PAITA. results demonstrated that Egr1, transcription factor, significantly overexpressed In Egr1 small interfering (si)RNA‑transfected cells, decreased compared negative control siRNA‑transfected indicating TLC‑S‑induced PAITA, overexpression enhanced ceRNA [mRNA‑microRNA (miRNA/miR)‑long non‑coding (lnc)RNA] generated using model revealed effects may be regulated by multiple pairs, lncRNAs (including NONRATT022624 NONRATT031002) miRNAs [including Rattus norvegicus (rno)‑miR‑214‑3p rno‑miR‑764‑5p] included pairs serve roles regulating Egr1. provide novel for researching of, developing treatments AP.