Diagnosis of the infection of sunflower by Orobanche cumana using multicolour fluorescence imaging

作者: María Luisa Pérez-Bueno , Matilde Barón , Ana Belén García-Carneros , Leire Molinero-Ruiz

DOI: 10.1515/HELIA-2014-0015

关键词:

摘要: Orobanche cumana is an holoparasite and thus totally dependent on sunflower for fixed carbon. Initial stages of the infection occur in first weeks after sowing are critical establishment a continuum between host parasite vascular system. From that moment obtains its supply water, mineral nutrients, assimilates from plant. Alterations plant metabolism can be detected using remote sensing techni- ques detection fluorescence emitted by plants. One these indirect techniques multicolour imaging. In this work, we assessed early O. ima- ging inferred physiological processes affected plants infected parasite. Ten germinated seeds inbred line NR5 were inoculated with population LP2013 cumana. The same number not was used as control. Sunflower planted pots soil mixture grown greenhouse at 12-22°C 6 weeks. Multicolour fluores- cence imaging conducted 3, 4, 5 inoculation. two pairs fully expanded leaves each imaged, and, measure date, five variables com- pared to those Three inoculation, when symptoms still observed, decreased levels blue green increased far-red observed inocu- lated At 4 displayed cumana, differences

参考文章(15)
F. P. C. Blamey, Richard K. Zollinger, Albert A. Schneiter, Sunflower Production and Culture Agronomy Monographs. pp. 595- 670 ,(2015) , 10.2134/AGRONMONOGR35.C12
M. Barón, J. Flexas, E.H. DeLucia, Terrestrial Photosynthesis in a Changing Environment: Photosynthetic responses to biotic stress Cambridge University Press. pp. 331- 350 ,(2012) , 10.1017/CBO9781139051477.026
Karl-Heinz Dammer, Bernd Möller, Bernd Rodemann, Dirk Heppner, Detection of head blight (Fusarium ssp.) in winter wheat by color and multispectral image analyses Crop Protection. ,vol. 30, pp. 420- 428 ,(2011) , 10.1016/J.CROPRO.2010.12.015
M L MOLINERO-RUIZ, B PÉREZ-VICH, R PINEDA-MARTOS, J M MELERO-VARA, Indigenous highly virulent accessions of the sunflower root parasitic weed Orobanche cumana Weed Research. ,vol. 48, pp. 169- 178 ,(2008) , 10.1111/J.1365-3180.2007.00611.X
J.M. Fernández-Martínez, J. Domínguez, B. Pérez-Vich, L. Velasco, Update on breeding for resistance to sunflower broomrape. Helia. ,vol. 33, pp. 1- 11 ,(2008) , 10.2298/HEL1052001F
J. M. Hibberd, W. P. Quick, M. C. Press, J. D. Scholes, Can source–sink relations explain responses of tobacco to infection by the root holoparasitic angiosperm Orobanche cernua? Plant Cell and Environment. ,vol. 21, pp. 333- 340 ,(1998) , 10.1046/J.1365-3040.1998.00272.X
Claus Buschmann, Hartmut K. Lichtenthaler, Principles and characteristics of multi-colour fluorescence imaging of plants Journal of Plant Physiology. ,vol. 152, pp. 297- 314 ,(1998) , 10.1016/S0176-1617(98)80144-2
María Luisa Pérez-Bueno, Mónica Pineda, Elena Díaz-Casado, Matilde Barón, Spatial and temporal dynamics of primary and secondary metabolism in Phaseolus vulgaris challenged by Pseudomonas syringae. Physiologia Plantarum. ,vol. 153, pp. 161- 174 ,(2015) , 10.1111/PPL.12237
Khadija Serghini, Alejandro Pérez de Luque, Mercedes Castejón‐Muñoz, Luis García‐Torres, Jesús V. Jorrín, Sunflower (Helianthus annuus L.) response to broomrape (Orobanche cernua Loefl.) parasitism: induced synthesis and excretion of 7‐hydroxylated simple coumarins Journal of Experimental Botany. ,vol. 52, pp. 2227- 2234 ,(2001) , 10.1093/JEXBOT/52.364.2227