Radiation and photografting as complementary techniques for immobilizing bioactive materials

摘要: Abstract The technique of radiation grafting to immobilize a typical enzyme, trypsin, three representative backbone polymers namely polypropylene, PVC and polystyrene, is described. process involves the simultaneous p-nitrostyrene polymer, nitro group subsequently being converted its isothiocyanato derivative which trypsin attached. For this immobilization work, utilization additives enhance shown be an advantage. Typical used are mineral acids (≈0.1M) polyfunctional monomers (≈1% v/v), two acting in synergistic fashion when together. A new class additives, metal salts such as LiClO 4 , also increase yields has now been discovered. reactivity these enhancement compared with that acid. model for modus operandi well proposed whereby increased attributed partitioning monomer into graft region presence ionic solutes. significance using prepare copolymers enzyme discussed. In addition ionizing radiation, UV value initiator preparation immobilization, however sensitizer copolymer may limit photografting method. possibility curing processes involving electron beam (EB) high powered (300 W/inch) sources work explored found feasible. system possesses great potential current since complete polymerization achieved fraction second.