Estimation of minimum electron dose necessary to resolve molecular structure of deoxyribonucleic acid by phase transmission electron microscopy
作者： Tsunenori Nomaguchi , Yoshihide Kimura , Yoshizo Takai
DOI: 10.1063/1.2402230
关键词:
摘要: The minimum electron dose that is necessary to resolve the molecular structure of deoxyribonucleic acid (DNA) was estimated based on experimental measurements information limits and simulated DNA images, considering conditions a low dose. From these results, ∼400e∕A2 found be achieve observation scale under present setup. A molecule observed by phase reconstruction method using through-focus images limited In reconstructed helical intervals base pairs were partially resolved.
scitation.org
aip.org 
sci-hub.se 参考文章(12)
John C. H. Spence, High-Resolution Electron Microscopy ,(2013)
SEM images of DNA double helix and nucleosomes observed by ultrahigh-resolution scanning electron microscopy. Journal of Electron Microscopy. ,vol. 40, pp. 181- 186 ,(1991) , 10.1093/OXFORDJOURNALS.JMICRO.A050893
Tadahiro Kawasaki, Yoshizo Takai, Takashi Ikuta, Ryuichi Shimizu, Wave field restoration using three-dimensional Fourier filtering method. Ultramicroscopy. ,vol. 90, pp. 47- 59 ,(2001) , 10.1016/S0304-3991(01)00122-X
Y. Fujiyoshi, N. Uyeda, Direct imaging of a double-strand DNA molecule Ultramicroscopy. ,vol. 7, pp. 189- 192 ,(1981) , 10.1016/0304-3991(81)90009-7
T. Kawasaki, M. Taya, T. Nomaguchi, Y. Takai, Effective phase correction function for high-resolution exit wave reconstruction by a three-dimensional Fourier filtering method. Ultramicroscopy. ,vol. 102, pp. 127- 139 ,(2005) , 10.1016/J.ULTRAMIC.2004.09.004
D. Van Dyck, A.F. de Jong, Ultimate resolution and information in electron microscopy: general principles Ultramicroscopy. ,vol. 47, pp. 266- 281 ,(1992) , 10.1016/0304-3991(92)90202-U
Y FUJIYOSHI, The structural study of membrane proteins by electron crystallography. Advances in Biophysics. ,vol. 35, pp. 25- 80 ,(1998) , 10.1016/S0065-227X(98)80003-8
A.F. de Jong, D. Van Dyck, Ultimate resolution and information in electron microscopy II. The information limit of transmission electron microscopes Ultramicroscopy. ,vol. 49, pp. 66- 80 ,(1993) , 10.1016/0304-3991(93)90213-H
Hiroyuki Tanaka, Chiho Hamai, Takashi Kanno, Tomoji Kawai, High-resolution scanning tunneling microscopy imaging of DNA molecules on Cu(111) surfaces Surface Science. ,vol. 432, ,(1999) , 10.1016/S0039-6028(99)00623-8
Takayuki Uchihashi, Masato Tanigawa, Makoto Ashino, Yasuhiro Sugawara, Kousuke Yokoyama, Seizo Morita, Mitsuru Ishikawa, Identification of B-Form DNA in an Ultrahigh Vacuum by Noncontact-Mode Atomic Force Microscopy Langmuir. ,vol. 16, pp. 1349- 1353 ,(2000) , 10.1021/LA991025I