Characterisation of Escherichia coli O157 isolates from Danish cattle and human patients by genotyping and presence and variants of virulence genes.

摘要: Escherichia coli O157 isolates obtained from 17 Danish cattle herds and a national surveillance programme of at slaughter were genotyped by pulsed-field gel electrophoresis (PFGE) characterised with respect to presence variation in virulence factors. The characteristics the strains compared human clinical same time period. All verocytotoxin (VT)-producing E. (VTEC O157) possessed all typical VTEC O157:H7 factors had either VT2c-variant alone or together VT1. Among dominant toxin profile was VT2+VT2c. Only one PFGE group represented on each farm, indicating that introduction establishment new these farms is probably not common. cattle, 22.8% VT-producing. majority eae gene other genotypic phenotypic traits for O157:H7. On basis characteristics, it concluded isolated are potential pathogens. However, observed differences between regard VT-profile, genotype antimicrobial resistance could be important, i.e. might most important reservoir infections mostly harbour less likely cause disease.