作者: Yawen Bai , Joan J. Englander , Leland Mayne , John S. Milne , S.Walter Englander
DOI: 10.1016/0076-6879(95)59051-X
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摘要: Just as exchangeable hydrogens that are controlled by global unfolding can be used to measure thermodynamic parameters at a level, exposed exchange local reactions may obtain locally resolved energy parameters. Results with the hemoglobin system demonstrate ability of HX methods locate functionally important changes in protein and energetic contribution each. These results offer promise measurements delineate, terms definable bonds their energies interactions, network interactions Hb other proteins use produce various functions.