Enhancement of antitumor effect using dendritic cells activated with natural killer cells in the presence of Toll-like receptor agonist

摘要: Dendritic cells (DCs) play a role in natural killer (NK) cell activation, while NK are also able to activate and mature DCs. Toll-like receptors (TLRs) on the surface of DCs induce maturation activation these when engaged with their cognate ligand. We investigated generate potent by presence TLR agonist vitro tested efficacy DC vaccinations mouse colon cancer model. The optimal ratios versus were 1:1 1:2. Immature lipopolysaccharide, which is TLR4 agonist, further addition IL-2 induced phenotypically functionally bone marrow-derived These exhibited not only high expression several costimulatory molecules production IL-12p40 IL-12p70, but allogeneic T stimulatory capacity, induction activities tumor-specific CTLs. Consistently, vaccination efficiently inhibited CT-26 tumor growth model compared other strategies. Interestingly, combination therapy DC-based vaccines low-dose cyclophosphamide showed dramatic inhibition effects growth. results suggest that maturated inducer antitumor immune responses may provide new source for development immunotherapy against cancer.