Metabolomic study of the response to cold shock in a strain of Pseudomonas syringae isolated from cloud water.

摘要: Active microorganisms have been recently discovered in clouds, thus demonstrating the capacity of to exist harsh environments, including exposure UV and oxidants, osmotic cold shocks, etc. It is important understand how respond survive such stresses at metabolic level. The objective this work assess metabolome modulation a strain Pseudomonas syringae isolated from cloud water facing temperature downshift 17 5 °C by identifying key molecules pathways response/adaptation shock. Bacterial extracts suspensions cells grown 17 °C further incubated microcosms 5 mimic conditions were analysed combining LC-MS NMR; results evaluated comparison similar kept constant temperature. differences profiles deciphered using multivariate statistics (PLS-DA). Key shock biomarkers observed, cryoprotectants (trehalose, glucose, glycerol, carnitine, glutamate), antioxidants (glutathione carnitine) their precursors, alkaloids (bellendine slaframine) metabolites involved energy metabolism (ATP, carbohydrates). Furthermore, new short peptides (nine dipeptides tetrapeptide) found that no known function. This study shows response temperatures, PDD-32b-74 demonstrates numerous modifications counteract impacts low temperatures.