Fimbria-mediated coaggregation between human oral anaerobes Treponema medium and Porphyromonas gingivalis.

摘要: Bacterial binding phenomena among different bacterial genera or species play an important role in colonization a mixed microbiota such as the human oral cavity. The coaggregation reaction between two gram-negative anaerobes, Treponema medium and Porphyromonas gingivalis, was characterized using fimbria-deficient mutants of P. gingivalis specific antisera against purified fimbriae whole cells. T. ATCC 700273 strongly coaggregated with fimbriate strains 33277 381, but not afimbriate including transposon-induced KDP98 fimA-disrupted mutant 33277. In gingivalis-T. assay, presence rabbit antiserum cells produced “aggregates” consisting predominantly few spirochetes, both preimmune serum did inhibit reaction. Heated lost their ability to bind heated unheated This medium-P. inhibited by cysteine proteinase inhibitor, leupeptin, also arginine lysine, EDTA sugars lactose. A assay on nitrocellulose membranes immunoelectron microscopy demonstrated that heat-stable 37 kDa surface protein cell attached fimbriae.