Manufacturing DNA microarrays of high spot homogeneity and reduced background signal.

摘要: Analyses on DNA microarrays depend considerably spot quality and a low background signal of the glass support. By using betaine as an additive to spotting solution made saline sodium citrate, both binding efficiency spotted PCR products homogeneity spots is improved significantly aminated surfaces such slides coated with widely used poly-l-lysine or aminosilane. In addition, non-specific markedly diminished. Concomitantly, during arraying procedure, reduces evaporation from microtitre dish wells, which hold products. Subsequent blocking chip surface succinic anhydride was in presence non-polar, non-aqueous solvent 1,2-dichloroethane acylating catalyst N-methylimidazole. This procedure prevents overall that occurs frequently applied aqueous 1-methyl-2-pyrrolidone borate buffer because re-dissolves process, only bind again across entire surface.