Sorf constructs and multiple gene expression

摘要: Embodiments of the invention relate to vector constructs and methods for expression polypeptides including multimeric products such as therapeutic antibodies. Particular allow generation from a single open reading frame (sORF). An embodiment provides an isolated or purified generating one more recombinant protein comprising insert; said insert signal peptide nucleic acid sequence encoding peptide; first polypeptide; intervening cleavage site, wherein site is provided by intein segment Ion protease gene Pyrococcus klbA Methanococcus, modified derived therefrom; second polypeptide. Certain embodiments employ abyssi, furiosus, horikoshii OT3; Methanococcus jannaschii; other segment.