Genome-wide analysis of the R2R3-MYB transcription factor genes in Chinese cabbage ( Brassica rapa ssp. pekinensis ) reveals their stress and hormone responsive patterns

摘要: The MYB superfamily is one of the most abundant transcription factor (TF) families in plants. proteins include highly conserved N-terminal repeats (1R, R2R3, 3R, and atypical) various C-terminal sequences that confer extensive functions. However, functions genes are unknown, have been little studied Chinese cabbage. Here, we analyzed 256 (55.2% total MYBs) R2R3-MYB from cabbage (Brassica rapa ssp. pekinensis) anchored them onto 10 chromosomes three subgenomes. R2R3-, 3R- atypical formed 45 subgroups based on domain similarity phylogenetic topology. Organization syntenic analysis revealed genomic distribution collinear relationships R2R3-BrMYBs. Synonymous nucleotide substitution (Ka/Ks) showed DNA-binding under strong purifying selection. Moreover, RNA-seq data tissue-specific distinct R2R3-BrMYB expression profiles, quantitative real-time PCR (qPCR) leaves stress responsive crosstalk with ABA-auxin signaling cascades. In this study, identified largest gene family plants to date. Our results indicate members may be involved plant development, responses leaf senescence, highlighting their functional diversity.