Polymorphonuclear leukocytes as a significant source of tumor necrosis factor-alpha in endotoxin-challenged lung tissue.

摘要: The kinetic expression and potential cellular source of tumor necrosis factor-alpha (TNF-alpha) in lipopolysaccharide-(LPS) induced acute lung inflammation was investigated using a rat model by Northern blot analysis, situ hybridization immunohistochemistry. LPS polymorphonuclear leukocyte infiltrate the that peaked between 6 24 hours. TNF-alpha messenger (m)RNA strongly whole tissues shown analysis. Both alveolar macrophages leukocytes (PMNs), purified from bronchoalveolar lavage fluids LPS-treated rats, were to express mRNA However, PMNs displayed several times more mRNA, relative actin than at 12 By hybridization, most cells positive for hours seemed be located within tissue near bronchioles or vessels. immunohistochemistry, protein localized mainly early (1 3 hours) after challenge, thereafter, predominant as 90% total intraalveolar PMN. Thus, our data provide first vivo evidence can serve significant sites inflammation.