An easy radioimmunological microassay of renin activity, concentration and substrate in human and animal plasma and tissues based on angiotensin I trapping by antibody.

摘要: ABSTRACT The principle of antibody-trapping angiotensin has allowed for the design an analysis performed entirely within one tube in a simple twostep procedure. first step is addition I antiserum to plasma. During incubation at 37 C controlled pH 7.4 formed captured and trapped by its antibody, giving protection against enzymatic conversion degradation. In order quantitate second large volume 125-I labeled 1 0 C. cooling dilution virtually stop reactions dissociates competes with antibody as other radioimmunoassays. expected versatility demonstrated detailed account on measurement renin concentration activity human rat plasma well methods determination substrate tissue renin. Minute amounts arerequired. Five μl suffice f...