Protein Expression Library Resources for Proteome Studies
作者： Joshua Labaer , Gerald Marsischky
DOI: 10.1016/B978-044451024-2/50028-X
关键词:
摘要: Publisher Summary There are several public projects worldwide with the goal of creating representative full-length cDNA clone collections, notably NIH Mammalian Gene Collection (MGC) project and Mouse Encyclopedia Project sponsored by RIKEN Institute in Japan. These have taken approach sifting through large numbers clones, e.g. from tissue-specific libraries, to identify unique clones. are, additionally, independent efforts create ORF collections for use as expression tools. include FLEXGene effort led Proteomics at Harvard Medical School (HIP), Caenorhabditis elegans ORFeome Project, Ressourcenzentrum fur Genomforschung (RZPD) collection. available this time either clones or While most straightforward create, they important limitations experimental The cloned sequences serve not only a tool assist assignment gene boundaries identification splice isoforms, but also starting point new kind genome-scale tool: an arrayed, sequence-validated, collection that is highly content organism. types under development human, mouse, model organisms, human pathogen, P. aeruginosa.
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