Intracellular metabolic changes in Saccharomyces cerevisiae and promotion of ethanol tolerance during the bioethanol fermentation process

摘要: During the batch bioethanol fermentation process, although Saccharomyces cerevisiae cells are challenged by accumulated ethanol, our previous work showed that ethanol tolerance of S. increased as time increased. However, exact molecular mechanisms underlying still poorly understood. In this study, a gas chromatography-mass spectrometry-based metabolomics strategy was used to determine process-associated intracellular metabolic changes in cells. With aid partial least squares-discriminant analysis between two three stages (i.e., lag, exponential, and stationary phases), 40 differential metabolites with variable importance projection value greater than 1 were identified. could continuously remodel their membrane composition structure obtain higher tolerance. lag–exponential phase transition, spite down-regulated TCA cycle, ergosterol content combined decreased saturated fatty acid might be most significant factor making yeast more robust ethanol-tolerant. exponential–stationary re-activated cycle provide plenty energy, energy production together requirements partly responsible for phase. Moreover, glycerol, trehalose, some amino acids also jointly confer These results highlighted knowledge about relationship process tolerance, contribute construction feasible ethanologenic strains