Scanning-fiber-based imaging method for tissue engineering
作者： Matthias C. Hofmann , Bryce M. Whited , Josh Mitchell , William C. Vogt , Tracy Criswell
DOI: 10.1117/1.JBO.17.6.066010
关键词:
摘要: A scanning-fiber-based method developed for imaging bioengineered tissue constructs such as synthetic carotid arteries is reported. Our approach based on directly embedding one or more hollow-core silica fibers within the scaffold to function micro-imaging channels (MIC). The process carried out by translating and rotating an angle-polished fiber micro-mirror MIC scan excitation light across scaffold. locally emitted fluorescent signals are captured using electron multiplying CCD camera then mapped into fluorophore distributions according positions. Using optical phantom composed of microspheres, scaffolds, porcine skin, we demonstrated single-cell-level resolution (20 30 μm) at depth that exceeds photon transport mean free path order magnitude. This result suggests no longer constrained scattering, but rather requirement signal overcomes background “noise” generated processes autofluorescence. Finally, compatibility our with engineering visualizing endothelial cells labeled green protein through a ∼500 μm thick highly scattering electrospun
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