Differential effects of chronic monocyte depletion on macrophage populations.

摘要: The administration of the bone-seeking isotope, 89Sr, to mice results in severe monocytopenia without any apparent effect on numbers resident peritoneal macrophages (Mphi). An explanation for this dichotomy was sought by determining whether residual blood monocytes were still an effective source Mphi after 89Sr treatment. Stem cell enumeration showed that a 90% fall bone marrow macrophage colony-forming cells accompanied 10-fold rise splenic M-CFC. Splenectomy performed before treatment, however, resulted little additional and had no affect even when sampling extended 31 days, interval beyond accepted half-time Mphi. Intraperitoneal injections thioglycollate or Corynebacterium parvum elicited few monocyte-Mphi during respective intervals 4 7 days. Elicitation with attempted tritiated thymidine-labeled 26 days 89Sr. Four later only 2-fold increase labeled found 89Sr-treated compared 150-fold controls. Studies ectoenzymes 5'-nucleotidase, alkaline phosphodiesterase I, leucine aminopeptidase such elicitation experiments suggested observed changes activities reflected direct stimulation rather than monocyte immigration. Overall, indicate treatment distinguishes two large populations basis their dependence marrow. inflammation reflect are severely rapidly depleted maintenance type Mphi, other hand, appears be independent both state level blood.