Reward for Bdellovibrio bacteriovorus for preying on a polyhydroxyalkanoate producer

摘要: Summary Bdellovibrio bacteriovorus HD100 is an obligate predator that invades and grows within the peri- plasm of Gram-negative bacteria, including mcl- polyhydroxyalkanoate (PHA) producers such as Pseudomonas putida. We investigated impact prey PHA content on fitness potential advantages for preying a producer. Using new procedure to control P. putida KT2442 cell size we demonstrated number Bdello- vibrio progeny depends biomass not viable or content. The presence mcl-PHA hydrolysed products in culture supernatant after predation KT42Z, producing strain lacking PhaZ depolymerase, confirmed ability degrade prey's PHA. Predator motility was higher when growing accumulating prey. External addition polymer (latex suspension) minus mutant KT42C1 restored movement, suggesting key component sustain swimming speed. High velocities observed were cor- related high intracellular ATP levels predator. These effects brought benefits more efficient than non-producing bacteria.