In vitro infection of woodchuck hepatocytes with woodchuck hepatitis virus and ground squirrel hepatitis virus.

摘要: Abstract Primary cultures of woodchuck hepatocytes were demonstrated to be susceptible in vitro infection by both hepatitis virus and ground squirrel virus, as evidenced the appearance DNA species characteristic hepadnavirus replication. Initiation was blocked presence suramin, polybrene, or dideoxycytidine. Viral CCC DNA, putative template for viral RNA transcription, detected at 2 days postinfection. Accumulation intracellular intermediates virion synthesis negligible until 7–10 postinfection, but these then increased dramatically amount over next few weeks. Results obtained which suggested that prolonged accumulation an intrinsic property individual cells, not result a slow spread through cultures.