Caspase-8 mediates caspase-3 activation and cytochrome c release during singlet oxygen-induced apoptosis of HL-60 cells.
作者： Shougang Zhuang , Mary C. Lynch , Irene E. Kochevar
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摘要: We reported previously that singlet oxygen, generated by irradiation of rose bengal with visible light, induced apoptosis in human promyelocytic leukemia HL-60 cells. However, the mechanism caused this reactive oxygen species is unclear. In study, we demonstrate caspase-3 activation and Z-DEVD-FMK, a inhibitor, blocked induction, while caspase-1 activity was not detectable inhibitor Z-YVAD-FMK had very limited effect on apoptosis. This suggests essential for commitment cells to undergo Further studies showed an increase caspase-8 reduction mitochondrial cytochrome c. Time course analysis indicated cleavage precedes caspase-3. addition, blockade Z-IETD-FMK inhibited pro-caspase-3 prevented loss These results suggest mediates c release during oxygen-induced
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