Ascorbate Peroxidase 1 Plays a Key Role in the Response of Arabidopsis thaliana to Stress Combination

作者: Shai Koussevitzky , Nobuhiro Suzuki , Serena Huntington , Leigh Armijo , Wei Sha

DOI: 10.1074/JBC.M806337200

关键词:

摘要: Within their natural habitat plants are subjected to a combination of different abiotic stresses, each with the potential exacerbate damage caused by others. One most devastating stress combinations for crop productivity, which frequently occurs in field, is drought and heat stress. In this study we conducted proteomic metabolic analysis Arabidopsis thaliana We identified 45 proteins that specifically accumulated response combination. These included enzymes involved reactive oxygen detoxification, malate metabolism, Calvin cycle. The accumulation malic enzyme during combined corresponded enhanced activity, decrease acid, lower amounts oxaloacetate, suggesting metabolism plays an important role Cytosolic ascorbate peroxidase 1 (APX1) protein mRNA When exposed drought, APX1-deficient mutant (apx1) more hydrogen peroxide was significantly sensitive than wild type. contrast, mutants deficient thylakoid or stromal/mitochondrial APXs were not apx1 Our findings suggest cytosolic APX1 key acclimation

参考文章(52)
Jörg Ziegler, Ivo Feussner, Claus Wasternack, Irene Stenzel, Bettina Hause, Otto Miersch, Tobias Kurz, Helmut Maucher, Heiko Weichert, Jasmonate biosynthesis and the allene oxide cyclase family of Arabidopsis thaliana. Plant Molecular Biology. ,vol. 51, pp. 895- 911 ,(2003) , 10.1023/A:1023049319723
R Mittler, B.A. Zilinskas, Molecular Cloning and Characterization of a Gene Encoding Pea Cytosolic Ascorbate Peroxidase Journal of Biological Chemistry. ,vol. 267, pp. 21802- 21807 ,(1992) , 10.1016/S0021-9258(19)36683-9
Elena Babiychuk, Sergei Kushnir, Enric Belles-Boix, Marc Van Montagu, Dirk Inzé, Arabidopsis thalianaNADPH Oxidoreductase Homologs Confer Tolerance of Yeasts toward the Thiol-oxidizing Drug Diamide Journal of Biological Chemistry. ,vol. 270, pp. 26224- 26231 ,(1995) , 10.1074/JBC.270.44.26224
Ludmila Rizhsky, Hongjian Liang, Ron Mittler, The Combined Effect of Drought Stress and Heat Shock on Gene Expression in Tobacco Plant Physiology. ,vol. 130, pp. 1143- 1151 ,(2002) , 10.1104/PP.006858
Thomas Stuhlfauth, Ralph Scheuermann, Heinrich P. Fock, Light Energy Dissipation under Water Stress Conditions: Contribution of Reassimilation and Evidence for Additional Processes. Plant Physiology. ,vol. 92, pp. 1053- 1061 ,(1990) , 10.1104/PP.92.4.1053
Ludmila Rizhsky, Hongjian Liang, Joel Shuman, Vladimir Shulaev, Sholpan Davletova, Ron Mittler, When Defense Pathways Collide. The Response of Arabidopsis to a Combination of Drought and Heat Stress Plant Physiology. ,vol. 134, pp. 1683- 1696 ,(2004) , 10.1104/PP.103.033431
Ron Mittler, Sandy Vanderauwera, Martin Gollery, Frank Van Breusegem, Reactive oxygen gene network of plants Trends in Plant Science. ,vol. 9, pp. 490- 498 ,(2004) , 10.1016/J.TPLANTS.2004.08.009