作者: Shai Koussevitzky , Nobuhiro Suzuki , Serena Huntington , Leigh Armijo , Wei Sha
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摘要: Within their natural habitat plants are subjected to a combination of different abiotic stresses, each with the potential exacerbate damage caused by others. One most devastating stress combinations for crop productivity, which frequently occurs in field, is drought and heat stress. In this study we conducted proteomic metabolic analysis Arabidopsis thaliana We identified 45 proteins that specifically accumulated response combination. These included enzymes involved reactive oxygen detoxification, malate metabolism, Calvin cycle. The accumulation malic enzyme during combined corresponded enhanced activity, decrease acid, lower amounts oxaloacetate, suggesting metabolism plays an important role Cytosolic ascorbate peroxidase 1 (APX1) protein mRNA When exposed drought, APX1-deficient mutant (apx1) more hydrogen peroxide was significantly sensitive than wild type. contrast, mutants deficient thylakoid or stromal/mitochondrial APXs were not apx1 Our findings suggest cytosolic APX1 key acclimation