Autophagic Vacuoles Produced In Vitro: I. Studies on Cultured Macrophages Exposed to Chloroquine

摘要: Mouse macrophages exposed to 30 µg/ml of chloroquine in vitro develop autophagic vacuoles containing various cytoplasmic components and acid phosphatase. The early toxic appear the perinuclear region within 15 min; on electron microscopy, they show irregular shape, amorphous moderately dense content, apparent double membranes, some instances curved thin tubular extensions with a central, dark linear element. Cytoplasmic structures are probably transported into by invagination vacuolar membrane. After exposure for 1–4 hr, display large degraded structures, membranous whorls, material. When is removed changing culture medium after 4 cells survive 24 hr later exhibit no abnormality except bodies packed membrane lamellae. During recovery disappears from cells. have accumulated less hydrolases than control