Diagnostic accuracy of in-house real-time PCR assay for Mycobacterium tuberculosis: a systematic review and meta-analysis.

摘要: In recent years, studies on the diagnostic accuracy of in-house real-time PCR (hRT-PCR) assay for detection Mycobacterium tuberculosis (Mtb) have been reported with unignorable discrepancies. To assess overall hRT-PCR Mtb diagnosis in different samples individuals active pulmonary and extra-pulmonary infection, a systematic review meta-analysis were performed. The PUBMED, EMBASE, Web Science, Cochrane databases searched up to June 2017 eligible that estimated sensitivity specificity respiratory non-respiratory infection patients, culture as reference standard. Bivariate random effect models used provide pooled estimation accuracy. Further, subgroup meta-regression analyses performed explore sources heterogeneity. risk bias was assessed by QUADAS-2 tool. Of 3589 candidate studies, 18 met our inclusion criteria. Compared data, 0.96 0.92, respectively. odds ratio (DOR) 192.96 (95% CI 68.46, 543.90), area under summary ROC curve (AUC) 0.9791. There significant heterogeneity among enrolled (p < 0.001). high-quality assessment application specimen associated better low-income/high-burden settings, results suggested could be useful test TB high specificity.