[13] Practical cryocrystallography.

摘要: Publisher Summary It is now practical to collect data routinely from macromolecular crystals at cryogenic temperatures. Crystals are prepared and rapidly cooled prevent ice-lattice formation in an aqueous medium. Instead of ice, a rigid glass forms, encasing the crystal with little or no damage. then maintained temperature around 100K during collection. This technique, known as “flash cooling,” offers number benefits that include minimization radiation-damage effects, reduction background scatter absorption, effective increase resolution limit, decrease thermal parameters for more ordered systems, capabilities long-term storage multiple reuses crystals. Practical considerations flash cooling collection temperatures discussed this chapter. Much technology experimental rationale crystallography were developed structure determination small inorganic organic compounds. Low-temperature also employed molecules disorder, reduce radiation damage, preserve metastable states, examine expansion coefficients phase transitions.