Identification of multiple HTF-island associated genes in the human major histocompatibility complex class III region.

摘要: Chromosome walking in the major histocompatibility complex (MHC) class III region has resulted isolation of 541 kb genomic DNA two sets overlapping cosmid clones. These encompass 340 separating C2 and tumour necrosis factor (TNF) alpha beta genes, except for a 22 gap 108 centromeric to TNF gene. The inserts have been characterized presence clusters restriction sites with CpG dinucleotides their recognition sequence. In conjunction pulsed field gel electrophoresis exact which cleave chromosomal established this suggested number HTF-islands. Genomic probes flanking HTF-islands hybridized Northern blots RNA from cell lines. Transcripts ranging size 0.6 6 corresponding products 12 novel, single copy genes identified. addition human equivalent murine B144 gene was mapped approximately 10 location so many new raises question as whether they play any role observed HLA associations an individual's susceptibility develop autoimmune disease.