The clinical utility of multimarker RT-PCR in the detection of occult metastasis in patients with melanoma.

摘要: Cutaneous melanoma is characterized by a high propensity for metastasis. Currently, surgical intervention remains the mainstay of therapy. This approach has proven most beneficial when diagnosis early stage primary lesions. Likewise, patients undergoing resection solitary site metastasis have shown survival advantage. Identification metastatic disease depends predominantly on radiographic techniques requiring presence significant tumor burdens successful imaging. However, at that time, role surgery and/or biochemotherapy may be limited value. Techniques to identify minimal states permit more accurate assessment prognosis. The detection occult cells RT-PCR in blood, lymph nodes, and bone marrow provides one such monitor progression. Single-marker been used as but noted limitations sensitivity specificity based heterogeneity marker expression among tumors well within an individual lesion or multiple lesions patients. We employed multimarker reverse transcriptase polymerase chain reaction assay demonstrates improved over single-marker approach. consequences detecting systemic subclinical remain unknown pending longer-term follow-up. using molecular conjunction with known clinicopathologic prognostic factors provide novel efficient monitoring progression further high-risk diagnosed course.