Microbeam MOMeNT: non-contact laser microdissection of membrane-mounted native tissue.

摘要: Abstract The analysis of tissue-specific genetic alterations depends on the selective procurement homogeneous cell populations. Microbeam microdissection membrane-mounted native tissue (MOMeNT) permits rapid, selective, and low-contamination tumor or other cells from histological sections by non-thermic non-contact laser microdissection. Tissue are mounted a specifically designed ultrathin transparent supporter membrane. together with membrane then dissected an ultraviolet (337-nm) pulsed microbeam coupled into robot-stage microscope. The causes dissection cold photolysis due to high photon density rather than local heating. track can be preselected freely video screen, size form dissectates thus adapted features section delineation accuracy in micron range. Polymerase chain reaction amplification DNA is not impaired, tumor-specific loss heterozygosity APC gene as well homozygous deletion MTS1 demonstrated bladder carcinomas. Taken together, MOMeNT novel technique that utilizes membrane-based microbeam, providing flexible, easy-to-use high-performance tool for molecular pathologist.