Estimation of kinetic cell-cycle-related gene expression in G1 and G2 phases from immunofluorescence flow cytometry data.

摘要: BACKGROUND Flow cytometry of immunofluorescence and DNA content provides measures cell-cycle-related gene expression (protein and/or epitope levels) for asynchronously growing cells. From these data, time-related through S phase can be directly measured. However, G1, G2, M phases, this information is unavailable. We present an objective method to model G1 G2 kinetic from estimate a minimum biological unit positive derived the distribution specific mitotic METHODS DU 145 cells were stained DNA, cyclin B1, marker (p105) analyzed by flow cytometry. The B1 (B1) p105-positive was used G1/S S/G2 interface measurements calculate in test validity approach. RESULTS at closely matched earliest modeled G2. increased linearly but exponentially G2; levels equivalent highest levels. modeling less certain than that due low demonstrated general feasibility. CONCLUSIONS By method, upper lower bounds could estimated modeled. Together with direct phase, throughout entire cell cycle should generally applicable given model-specific assumptions.