Preparative Purification of Puerarin from Pueraria Flavones by Oligo‐β‐Cyclodextrin‐Sepharose HP Matrix
作者： Manyi Wang , Weina Kan , Xiangling He , Tianwei Tan , Janson Jan‐Christer
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摘要: Abstract The matrix oligo‐β‐cyclodextrin‐Sepharose HP was synthesized and successfully applied in the separation of puerarin from pueraria flavones. optimal conditions preparation were obtained. carried out with 7% acetic acid solution (V %) at a flow rate 10 mL/min, after 300 mg flavones 20 mL 5% ethanol injected preparative column (350 mm×44 mm i.d.). fraction collected further purification performed. purity obtained higher than 97%. recovery whole procedure reached 32.4%.
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