Development of Inherently Echogenic Liposomes as an Ultrasonic Contrast Agent

摘要: Abstract Ultrasonic contrast agents have been developed for improved assessment of blood flow and tissue perfusion. Many these are not inherently acoustically reflective (echogenic), nearly all suitable specific targeting. The purpose this study was to develop liposomes, which antibody conjugation, without using gas or any other agent entrapment. Echogenic liposomes were prepared from phosphatidylcholine (PC), phophatidyl-ethanolamine (PE), phosphatidylglycerol (PG), cholesterol (CH), a dehydration/rehydration method. formulation optimized higher acoustic reflectivity by varying the lipid composition. Liposomes imaged with 20 MHz intravascular ultrasonic imaging catheter. Echogenicity levels expressed pixel gray scale. presence PE PG at concentrations echogenicity due their effects on liposomal morphology as confirmed freeze-etch electron microscopy. retained when treated room temperature 37°C under in vitro conditions. It demonstrated that also vivo after they injected into miniswine model. We echogenic stable targeting novel agent. This new can be used image enhancement and/or treatment targeted pathologic sites.