Food preparation with mucoralean fungi: A potential biosafety issue?

摘要: Mucorales have been used for production of fermented food in Asia and Africa since time immemorial. Particularly Rhizopus species are rapidly growing, active producers lipases proteases occur naturally during the first stages soybean fermentation. Two biosafety issues raised recent literature: (1) pathogenicity, being prevalent opportunists causing erosive infections severely compromised patients, (2) toxicity, strains harbouring endosymbiotic Burkholderia producing toxic secondary metabolites. At molecular level, based on different gene markers, identity was found between processing clinical strains. In this study, we screened bacterial symbionts 64 by light microscopy, 16S rRNA sequencing, HPLC. Seven (11 %) carried bacteria identified as rhizoxinica endofungorum, an unknown species. The isolates proved to be able produce rhizoxins. Strains with endosymbionts originated from food, soil, a source, thus their presence could not linked particular habitats. toxins excluded potential risk human health. contrast, given type diseases caused species, regard practical infection via industry negligible.