Longitudinal study of circulating protein biomarkers in inflammatory bowel disease

摘要: Abstract Inflammatory bowel diseases (IBDs) are chronic and progressive inflammatory disorders of the gastrointestinal tract. In IBD, protein serological biomarkers could be relevant tools for assessing disease activity, performing early-stage diagnosis managing treatment. Using interleukin-10 knockout (IL-10 −/− ) mouse, a model that develops time-dependent IBD-like disorder predominates in colon; we performed longitudinal studies circulating IBD. Circulating profiles serum samples collected from 30-, 93-, to 135-day-old IL-10 mice were investigated using two-dimensional differential gel electrophoresis MALDI-TOF/TOF tandem mass spectrometry. A total 15 different proteins identified confirmed by ELISA Western blot differentially accumulated mid- late-stage compared early non-inflamed mice. The use another colitis an extra-intestinal inflammation validated this biomarker panel demonstrated comprised some global markers, intestinal inflammation-specific markers markers. Statistical analyses misclassification error rate charts these as powerful colitis. Unlike standard screening studies, our allowed definition signatures reflect status. Biological significance Crohn's (CD) ulcerative (UC) most common occurring humans. major current tool is colonoscopy, which invasive lead false diagnosis. emergence enables new such IBD diagnosis/management. 2D-DIGE coupled spectrometry, study mouse signature specific stages disease.