SDF-1/CXCR4-mediated migration of transplanted bone marrow stromal cells toward areas of heart myocardial infarction through activation of PI3K/Akt.

摘要: Abstract Stromal cell-derived factor-1 (SDF-1) and its receptor, CXCR4, are crucial for homing migration of multiple stem cell types. Their potential role in mediating bone marrow-derived mesenchymal (BMSC) areas myocardial infarction (MI) has not been demonstrated. In this study, rat heart MI was created by left coronary artery ligation, green fluorescent protein-labeled BMSCs were directly infused into the ventricular cavity. Reverse transcriptase-polymerase chain reaction Western blot analysis showed that SDF-1 predominantly localized lesion, levels peaked 3 to 7 days maintained at least 14 days. Additionally, matched with increased accumulation an improvement cardiac function. Furthermore, effect blocked phosphoinositide 3-kinase inhibitor, LY294002. vitro experiments CXCR4 expression elevated during hypoxia induced a concentration-dependent BMSCs. This CXCR4-dependent as confirmed total inhibition AMD3100, CXCR4-specific antagonist. Migration also almost completely Analysis phosphorylated Akt highly SDF-1-treated Together these results demonstrated SDF-1/CXCR4 may mediate toward through activation PI3K/Akt.