Transglutaminase crosslinked gelatin as a tissue engineering scaffold

摘要: Gelatin is one of the most commonly used biomaterials for creating cellular scaffolds due to its innocuous nature. In order create stable gelatin hydrogels at physiological temperatures (37 degrees C), chemical crosslinking agents such as glutaraldehyde are typically used. To circumvent potential problems with residual amounts these crosslinkers in vivo and that both physiologically robust biocompatible, a microbial transglutaminase (mTG) was this study enzymatically crosslink solutions. HEK293 cells encapsulated mTG-crosslinked proliferated rate 0.03 day(-1). When released via proteolytic degradation trypsin, were able recolonize tissue culture flasks, suggesting therapeutic purposes could be delivered using an construct. Upon submersion saline solution 37 C, exhibited no mass loss, within experimental error, indicating material thermally stable. The RT slightly faster than thermally-cooled (physically-crosslinked) gelatin. Thermally-cooled subsequently crosslinked mTG resulted more resistant proteolysis. Degradation rates found tunable content, attribute may useful either long-time cell encapsulation or time-released regenerative delivery. Further investigation showed controlled by surface erosion.