Limb ischemic preconditioning mitigates lung injury induced by haemorrhagic shock/resuscitation in rats

摘要: Abstract Aim of the study Haemorrhagic shock and subsequent resuscitation induce acute lung injury. We elucidated whether bilateral lower limb ischemic pre-conditioning (IP) could mitigate injury in haemorrhagic shock/resuscitation rats. The role heme oxygenase-1 (HO-1) was also elucidated. Method Adult male rats were randomized to receive (HS), HS plus IP, or IP HO-1 inhibitor tin protoporphyrin (SnPP) ( n =12 each group). Sham groups employed simultaneously. For pre-conditioning, 3 cycles (10min ischemia followed by 10min reperfusion) performed immediately before shock. (mean arterial pressure: 40–45mmHg) induced blood drawing maintained for 120min. SnPP injected 5min resuscitation. Shed blood/saline mixtures re-infused achieve After monitoring another 8h, sacrificed. Arterial gas alveolar–arterial oxygen difference (lung function index), histology, polymorphonuclear leukocytes/alveoli ratio (leukocyte infiltration wet/dry weight (water content inflammatory molecules (e.g., chemokine, cytokine, prostaglandin E 2 ), malondialdehyde (lipid peroxidation index) assays preformed. Results significant alterations increases leukocyte infiltration, water content, inflammation, lipid lungs. Histological analysis confirmed that caused marked Limb significantly mitigated adverse effects shock/resuscitation. Moreover, protective reversed SnPP. Conclusions mitigates mechanisms may involve HO-1.