The Loop 2 Region of Ribosomal Protein uS5 Influences Spectinomycin Sensitivity, Translational Fidelity, and Ribosome Biogenesis.

摘要: Ribosomal protein uS5 is an essential component of the small ribosomal subunit that involved in assembly, maintenance translational fidelity, and ribosome's response to antibiotic spectinomycin. While many characterized mutations affect decoding map its interface with uS4, more recent work has shown residues distant from uS4-uS5 can also process. We targeted one such interface-remote area, loop 2 region (residues 20 31), for mutagenesis Escherichia. coli generated 21 unique mutants. A majority alterations confer resistance spectinomycin fidelity translation. However, only a minority show altered rRNA processing or ribosome biogenesis defects.