Quantitation of human papillomavirus 16 E6 and E7 DNA and RNA in residual material from ThinPrep Papanicolaou tests using real-time polymerase chain reaction analysis.

摘要: BACKGROUND The detection of specific human papillomavirus 16 (HPV-16) E6 and E7 oncogene transcripts may be a sensitive indicator the direct involvement viral oncogenes in development cervical neoplasia carcinoma. The goal this study was to determine potential clinical uses real-time polymerase chain reaction (PCR) reverse transcription-PCR (RT-PCR) methods for evaluating HPV-16 expression. METHODS ThinPrep samples were tested expression by PCR or RT-PCR analysis compared with conventional analysis. Both sets results correlated cytologic diagnosis samples. RESULTS The presence DNA RNA observed only positive carcinoma cell lines but not HPV-18 HPV negative lines. percentage series ThinPrep (n = 348 samples) 0% 45 samples), 9.7% atypical squamous cells undetermined significance (ASCUS; n 144 16.9% low-grade intraepithelial lesion (LSIL; 118 51.2% high-grade (HSIL; 41 samples). copy numbers per nanogram both increased significantly as severity lesions progressed from ASCUS HSIL, more than numbers. increase took place stepwise fashion ASCUS, LSIL, HSIL. CONCLUSIONS The specimens serve quick, reliable, tool identify subset patients who express oncoproteins. Cancer 2002;94:2199–210. © 2002 American Society. DOI 10.1002/cncr.10439