DNA lesion identity drives choice of damage tolerance pathway in murine cell chromosomes
作者： Isadora S. Cohen , Carmit Bar , Tamar Paz-Elizur , Elena Ainbinder , Karoline Leopold
DOI: 10.1093/NAR/GKU1398
关键词:
摘要: DNA-damage tolerance (DDT) via translesion DNA synthesis (TLS) or homology-dependent repair (HDR) functions to bypass lesions encountered during replication, and is critical for maintaining genome stability. Here, we present piggyBlock, a new chromosomal assay that, using piggyBac transposition of containing known lesion, measures the division labor between two DDT pathways. We show that in absence damage response, most common sunlight-induced TT-CPD, achieved by TLS mouse embryo fibroblasts. Meanwhile, BP-G, major smoke-induced bypassed primarily HDR, providing first evidence this mechanism being main pathway biologically important lesion mammalian genome. also far from last-resort strategy as it sometimes portrayed, operates alongside nucleotide excision repair, handling 40% TT-CPDs repair-proficient cells. Finally, acts embryonic stem cells, exhibiting same pattern—mutagenic included—despite risk propagating mutations along all cell lineages. The method highlights importance provides an effective tool studying
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