Rapid Purification of 70S RNA from Media of Cells Producing RNA Tumor Viruses

摘要: Abstract Media from cells producing RNA tumor viruses, when treated with sodium dodecyl sulfate and polyvinyl sulfate, yield 70S as the major species binding oligo(dT)-cellulose. The procedure described for purifying requires no special equipment is suitable rapidly processing large quantities of media or commercially available virus, a 5- to 10-fold higher than was obtained using existing methods.