Generation of diversity in nonerythroid spectrins. Multiple polypeptides are predicted by sequence analysis of cDNAs encompassing the coding region of human nonerythroid alpha-spectrin.
作者： R T Moon , A P McMahon
DOI: 10.1016/S0021-9258(19)39582-1
关键词:
摘要: Nonerythroid alpha-spectrin (alpha-fodrin) is a major component of the membrane skeleton in diverse cell types. Overlapping cDNAs have been isolated which encompass coding region human lung fibroblast nonerythroid alpha-spectrin. The composite sequence 7,787 nucleotides encodes polypeptide 2,472 amino acids (predicted Mr 283,964). This has 58% acid identity with erythroid alpha-spectrin, encoded on different gene, and 96% full-length chicken brain We previously reported variable expression 20 between repeats 10 11 (McMahon, A. P., Giebelhaus, D. H., Champion, J. E., Bailes, A., Lacey, S., Carritt, B., Henchman, S. K., Moon, R. T. (1987) Differentiation 34, 68-78). In this study, we report additional heterogeneity near carboxyl-terminal end. One (clone 3D) an in-frame deletion 18 within spectrin repeat 21 when compared to overlapping cDNA 7). As occurs domains suggested bind calcium or actin, it possible that fibroblasts express functionally distinct isoforms
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