Rapid identification of flaviviruses based on conserved NS5 gene sequences
作者： L. Fulop , A.D.T. Barrett , R. Phillpotts , K. Martin , D. Leslie
DOI: 10.1016/0166-0934(93)90053-T
关键词:
摘要: Two conserved regions in the sequence of NS5 gene Flaviviruses were identified. Primers designed from consensus these and used a reverse transcription/polymerase chain reaction (RT/PCR) to amplify region central european tick-borne encephalitis virus Kumlinge gene. The authenticity amplified fragment was confirmed by nucleotide sequencing. A band expected size also obtained when this RT/PCR applied 13 other flaviviral RNAs. This method may be useful for characterisation genes flaviviruses as potential pan-flavivirus diagnostic tool.
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