Acetate uptake by PHA-accumulating and non-PHA-accumulating organisms in activated sludge from an aerobic sequencing batch reactor fed with acetate

摘要: The present study was conducted to evaluate the specific acetate uptake rates of microorganisms with and without polyhydroxyalkanoates (PHA) accumulation. Activated sludge aerobically incubated 75 mgC L −1 radiolabeled or non-labeled acetate, consumption PHA accumulation were monitored. Microorganisms quantified as follows: all microbial cells by DAPI staining, whole utilizing organisms microautoradiography, PHA-accumulating staining Nile blue A. abundance acetate-utilizing also calculated from outcomes. estimate utilized PHAAOs included both converted that used supply reducing power ATP. Acetate non-PHAAOs divided their respective abundances obtain rates: ranged between 5.3 8.0 × 10 −10  mgC cell  h , 2.8 4.2 × 10 .