Profiling the immunome of little brown myotis provides a yardstick for measuring the genetic response to white-nose syndrome.

摘要: White-nose syndrome (WNS) has devastated populations of hibernating bats in eastern North America, leading to emergency conservation listings for several species including the previously ubiquitous little brown myotis (Myotis lucifugus). However, some bat near epicenter WNS panzootic appear be stabilizing after initial precipitous declines, which could reflect a selective immunogenetic sweep. To investigate hypothesis that exerts significant selection on immunome affected populations, we developed novel, high-throughput sequence capture assay targeting 138 adaptive, intrinsic, and innate immunity genes putative adaptive significance, as well their respective regulatory regions (~370 kbp genomic sequence/individual). We used explore baseline variation M. lucifugus whether particular immune genes/variants are associated with susceptibility. also our detect 1,038 putatively neutral single nucleotide polymorphisms characterize contemporary population structure, providing context identification local adaptation. Sequence provided cost-effective, "all-in-one" test genetic structure revealed fine-scale, differentiation between sampling sites <600 km apart. identified functional variants This study lays foundations future investigations rangewide adaptation provides blueprint studies evolutionary rescue other host-pathogen systems.