Enhanced expression of Heat shock proteins in activated polymorphonuclear leukocytes in patients with sepsis
作者： Naoyuki Hashiguchi , Hiroshi Ogura , Hiroshi Tanaka , Taichin Koh , Yasushi Nakamori
DOI: 10.1097/00005373-200112000-00015
关键词:
摘要: Background: Heat shock proteins (HSPs) in cells, as molecular chaperons, have been reported to regulate cell functions. The objective of this study was investigate the HSP expression polymorphonuclear leukocytes (PMNLs) from severe septic patients and relation between HSPs PMNL function. Methods: In blood samples 21 with sepsis serum C-reactive protein levels more than 10 mg/dL, we used flow cytometry measure expressions HSP27, HSP60, HSP70, HSP90; oxidative activity; apoptosis PMNLs during sepsis. vitro studies, cells 14 healthy volunteers examine HSP70 Quercetin (30 μM), a suppressor HSP, sodium arsenite (100 an inducer were PMNLs, activity these measured. Results: sepsis, HSP90 significantly increased. Apoptosis markedly inhibited. administration enhanced increased activity, inhibited apoptosis. Administration quercetin before prevented increase inhibition Conclusion: Sepsis causes activated PMNLs. is may play role regulating function
sci-hub.se 参考文章(36)
F Colotta, F Re, N Polentarutti, S Sozzani, A Mantovani, Modulation of granulocyte survival and programmed cell death by cytokines and bacterial products Blood. ,vol. 80, pp. 2012- 2020 ,(1992) , 10.1182/BLOOD.V80.8.2012.2012
G. C. Tsokos, S.-N. C. Liossis, J. G. Kiang, X. Z. Ding, Overexpression of the heat shock protein 70 enhances the TCR/CD3- and Fas/Apo-1/CD95-mediated apoptotic cell death in Jurkat T cells. Journal of Immunology. ,vol. 158, pp. 5668- 5675 ,(1997)
Hideki Fukata, Atsushi Uchida, Yoshitaka Kariya, Keisuke Teshigawara, Yu-quan Wei, Xia Zhao, Induction of apoptosis by quercetin: involvement of heat shock protein. Cancer Research. ,vol. 54, pp. 4952- 4957 ,(1994)
A. J. Ryan, S. W. Flanagan, P. L. Moseley, C. V. Gisolfi, Acute heat stress protects rats against endotoxin shock. Journal of Applied Physiology. ,vol. 73, pp. 1517- 1522 ,(1992) , 10.1152/JAPPL.1992.73.4.1517
J W Parce, M C Seeds, D A Bass, P Szejda, M Thomas, L R Dechatelet, Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. Journal of Immunology. ,vol. 130, pp. 1910- 1917 ,(1983)
T. Langer, W. Neupert, Heat shock proteins hsp60 and hsp70: their roles in folding, assembly and membrane translocation of proteins. Current Topics in Microbiology and Immunology. ,vol. 167, pp. 3- 30 ,(1991) , 10.1007/978-3-642-75875-1_1
Naoyuki Hashiguchi, Hiroshi Ogura, Hiroshi Tanaka, Taichin Koh, Masayuki Aoki, Tadahiko Shiozaki, Tetsuya Matsuoka, Takeshi Shimazu, Hisashi Sugimoto, Enhanced expression of heat shock proteins in leukocytes from trauma patients. Journal of Trauma-injury Infection and Critical Care. ,vol. 50, pp. 102- 107 ,(2001) , 10.1097/00005373-200101000-00018
Shauna M. Hennigan, Jiang Huai Wang, H. Paul Redmond, David Bouchier-Hayes, Neutrophil heat shock protein expression and activation correlate with increased apoptosis following transmigration through the endothelial barrier. Shock. ,vol. 12, pp. 32- 38 ,(1999) , 10.1097/00024382-199907000-00005
I. Nicoletti, G. Migliorati, M.C. Pagliacci, F. Grignani, C. Riccardi, A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry Journal of Immunological Methods. ,vol. 139, pp. 271- 279 ,(1991) , 10.1016/0022-1759(91)90198-O
IAN D. CHANT, PETER E. ROSE, ALAN G. MORRIS, Analysis of heat-shock protein expression in myeloid leukaemia cells by flow cytometry. British Journal of Haematology. ,vol. 90, pp. 163- 168 ,(1995) , 10.1111/J.1365-2141.1995.TB03395.X