A high-resolution interactive atlas of the human brainstem using magnetic resonance imaging.

摘要: Abstract Conventional atlases of the human brainstem are limited by inflexible, sparsely-sampled, two-dimensional nature histology, or low spatial resolution conventional magnetic resonance imaging (MRI). Postmortem high-resolution MRI circumvents challenges associated with both modalities. A single specimen extending from rostral diencephalon through caudal medulla was prepared for after brain removed a 65-year-old male within 24 h death. The formalin-fixed two weeks, then rehydrated and placed in custom-made compatible tube immersed liquid fluorocarbon. performed 7-Tesla scanner 120 unique diffusion directions. Acquisition time anatomic images were 14 h 208 h, respectively. Segmentation manually. Deterministic fiber tractography done using strategically chosen regions interest avoidance, manual editing expert knowledge neuroanatomy. Anatomic rendered isotropic resolutions 50 μm 200 μm, Ninety different structures segmented labeled, 11 bundles tractography. complete atlas is available online interactive use at https://civmvoxport.vm.duke.edu/voxbase/login.php?return_url=%2Fvoxbase%2F . This presents multiple contrasting datasets selected tract reconstruction unprecedented MR brainstem. There immediate applications neuroanatomical education, potential to serve future research enhanced neurosurgical planning “safe” zones entry into