Interactions of silver nanoparticles with primary mouse fibroblasts and liver cells.

摘要: Primary cells are ideal for in vitro toxicity studies since they closely resemble tissue environment. Here, we report a detailed study on the interactions of 7-20 nm spherical silver nanoparticles (SNP) with primary fibroblasts and liver isolated from Swiss albino mice. The intended use is form topical antimicrobial gel formulation treatment burns wounds. Upon exposure to SNP 24 h, morphology remained unaltered up 25 microg/mL 100 SNP, respectively, although minor decrease confluence. IC(50) values as revealed by XTT assay were 61 449 microg/mL, respectively. Ultra-thin sections exposed 1/2 visualized under Transmission electron microscope showed presence dark, dense, aggregates inside mitochondria, cytoplasm, probably representing intracellular SNP. When challenged approximately concentration (i.e. 30 225 cells, respectively), enhancement GSH (approximately 1.2 fold) depletion lipid peroxidation 1.4 seen which protect functional damage. In case cells; increased levels SOD ( 1.1 compared unexposed observed. Caspase-3 activity indicated that concentrations required onset apoptosis found be much lower (3.12 fibroblasts, 12.5 cells) than necrotic (100 500 cells). These observations confirmed CLSM (resulting apoptosis) 2 x IC(50)) necrosis). results clearly suggest seem enter eukaryotic cellular antioxidant mechanisms possible oxidative This property, conjunction finding possess higher tolerance 20 microg/g), indicates preliminary safety warrants further human application.